He had a lot of real estate and some material possessions. He named, in this order:
- Son Joseph
- Grandson Reuben (son of John)
- Daughter-in-law Jean (my records have Jane), widow of Tristram, Jr.
- Tristram Jr.'s children: John, Jean, Reuben (again), and Tristram III.
- Grandchildren Mary and Nathaniel, children of his son Nathaniel.
- Son John
- Wife Abigail
- Son Samuel
- Daughters Elizabeth Knight, Mary Warren, and Keziah Wentworth.
- Grandsons Joseph Knight and Tristram Warren
- Wife Abigail (again). He made her and their son Samuel his co-executors.
His will is very long. The transcription is in his gallery.